Difference between revisions of "Part:BBa K2324012"

 
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<partinfo>BBa_K2324012 short</partinfo>
 
<partinfo>BBa_K2324012 short</partinfo>
  
This part contains the <i> fim operon </i> under control of a constitutive promoter. When co-transformed with the other parts from our project, which have rhamnose-inducible promoters, it will help to synthesise modified type I pilus structures. The part requires no induction, and therefore is able to continuously produce the large mass of protein encoded in the operon.  
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This part contains the <i> fim operon </i> under control of a constitutive promoter. When co-transformed with FimH constructs from our project, which have rhamnose-inducible promoters, modified type I pili may be produced. The part requires no induction, and therefore is able to continuously produce the large mass of protein encoded in the operon.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 18:12, 1 November 2017


pJ23100_FimOp

This part contains the fim operon under control of a constitutive promoter. When co-transformed with FimH constructs from our project, which have rhamnose-inducible promoters, modified type I pili may be produced. The part requires no induction, and therefore is able to continuously produce the large mass of protein encoded in the operon.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2648
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 931
    Illegal AgeI site found at 962
  • 1000
    COMPATIBLE WITH RFC[1000]