Difference between revisions of "Part:BBa K2443003"
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− | Aspartic acid tRNA synthetase responsible for attaching aspartic acid onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in <i>Escherichia coli</i>. Contains a C-terminal | + | Aspartic acid tRNA synthetase responsible for attaching aspartic acid onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in <i>Escherichia coli</i>. Contains a C-terminal hexahistidine tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator. AspRS has an expected size of 67 kDa, which corresponds to the band size seen in lanes 2 and 3 below. Protein was overexpressed from BL21DE3 gold where the construct was in pSB1C3. Overexpression was performed according to the overexpression protocols, and notebook on the 2017 iGEM Lethbridge wiki (https://static.igem.org/mediawiki/2017/2/2c/Protein_Overexpression_.pdf and https://static.igem.org/mediawiki/2017/0/0d/October_.pdf). |
+ | https://static.igem.org/mediawiki/parts/d/d4/Lethbridge_2017_Oct_10_gel_2.png | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
− | <partinfo> | + | <partinfo>BBa_K2443003 SequenceAndFeatures</partinfo> |
Latest revision as of 18:11, 1 November 2017
AspRS optimized for expression in E. coli
Aspartic acid tRNA synthetase responsible for attaching aspartic acid onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in Escherichia coli. Contains a C-terminal hexahistidine tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator. AspRS has an expected size of 67 kDa, which corresponds to the band size seen in lanes 2 and 3 below. Protein was overexpressed from BL21DE3 gold where the construct was in pSB1C3. Overexpression was performed according to the overexpression protocols, and notebook on the 2017 iGEM Lethbridge wiki (https://static.igem.org/mediawiki/2017/2/2c/Protein_Overexpression_.pdf and https://static.igem.org/mediawiki/2017/0/0d/October_.pdf).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 835
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 752