Difference between revisions of "Part:BBa K2460004:Design"
| Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | + | *Note that if you are using a repressor to regulate the integrase, make sure there are already repressors accumulated in the cell to prevent integrase from expressing. | |
| + | *An attB/P site follwing after a promoter may reduce experssion. | ||
| + | *This part has a 6×His tag and a thrombin site, and mutated to be RFC10 compatible. | ||
| Line 13: | Line 15: | ||
===Source=== | ===Source=== | ||
| − | + | Lab storage | |
===References=== | ===References=== | ||
Latest revision as of 18:10, 1 November 2017
φRv1 integrase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 274
Illegal NgoMIV site found at 391
Illegal NgoMIV site found at 422
Illegal NgoMIV site found at 958
Illegal AgeI site found at 1364 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
- Note that if you are using a repressor to regulate the integrase, make sure there are already repressors accumulated in the cell to prevent integrase from expressing.
- An attB/P site follwing after a promoter may reduce experssion.
- This part has a 6×His tag and a thrombin site, and mutated to be RFC10 compatible.
Source
Lab storage