Difference between revisions of "Part:BBa K2443001"

Latest revision as of 18:05, 1 November 2017

ArgRS optimized for expression in E. coli

Arginine tRNA synthetase responsible for attaching asparagine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in Escherichia coli. Contains a C-terminal hexahistidine tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator. The expected size of ArgRs is 66 kDa, which corresponds with the band size shown in lane 5 in the overexpression gel below. Protein was overexpressed from BL21DE3 gold from pSB1C3 according to the protocols and notebook listed on the iGEM 2017 Lethbridge wiki (https://static.igem.org/mediawiki/2017/2/2c/Protein_Overexpression_.pdf and https://static.igem.org/mediawiki/2017/0/0d/October_.pdf)

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Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1805
Illegal SapI.rc site found at 1523

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 <partinfo>BBa_K2443001 short</partinfo>
-Arginine tRNA synthetase responsible for attaching asparagine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in <i>Escherichia coli</i>. Contains a C-terminal histidine tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator.
+Arginine tRNA synthetase responsible for attaching asparagine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in <i>Escherichia coli</i>. Contains a C-terminal hexahistidine tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator. The expected size of ArgRs is 66 kDa, which corresponds with the band size shown in lane 5 in the overexpression gel below. Protein was overexpressed from BL21DE3 gold from pSB1C3 according to the protocols and notebook listed on the iGEM 2017 Lethbridge wiki (https://static.igem.org/mediawiki/2017/2/2c/Protein_Overexpression_.pdf and https://static.igem.org/mediawiki/2017/0/0d/October_.pdf)
+"https://static.igem.org/mediawiki/parts/e/e3/BBa_K2443001-Oct_17_gel1.png"