Difference between revisions of "Part:BBa K2443001"
 
(3 intermediate revisions by 3 users not shown)
Line 3: Line 3:
 
<partinfo>BBa_K2443001 short</partinfo>
 
<partinfo>BBa_K2443001 short</partinfo>
  
−
Arginine tRNA synthetase responsible for attaching asparagine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in <i>Escherichia coli</i>. Contains a C-terminal histidine tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator.  
+
Arginine tRNA synthetase responsible for attaching asparagine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in <i>Escherichia coli</i>. Contains a C-terminal hexahistidine tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator. The expected size of ArgRs is 66 kDa, which corresponds with the band size shown in lane 5 in the overexpression gel below. Protein was overexpressed from BL21DE3 gold from pSB1C3 according to the protocols and notebook listed on the iGEM 2017 Lethbridge wiki (https://static.igem.org/mediawiki/2017/2/2c/Protein_Overexpression_.pdf and https://static.igem.org/mediawiki/2017/0/0d/October_.pdf)
 +
 
 +
"https://static.igem.org/mediawiki/parts/e/e3/BBa_K2443001-Oct_17_gel1.png"
 +
 
  
  
Line 11: Line 14:
 
<!-- -->
 
<!-- -->
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
−
<partinfo>BBa_K2443003 SequenceAndFeatures</partinfo>
+
<partinfo>BBa_K2443001 SequenceAndFeatures</partinfo>
  
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
−
<partinfo>BBa_K2443003 parameters</partinfo>
+
<partinfo>BBa_K2443001 parameters</partinfo>
 
<!-- -->
 
<!-- -->

Latest revision as of 18:05, 1 November 2017


ArgRS optimized for expression in E. coli

Arginine tRNA synthetase responsible for attaching asparagine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in Escherichia coli. Contains a C-terminal hexahistidine tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator. The expected size of ArgRs is 66 kDa, which corresponds with the band size shown in lane 5 in the overexpression gel below. Protein was overexpressed from BL21DE3 gold from pSB1C3 according to the protocols and notebook listed on the iGEM 2017 Lethbridge wiki (https://static.igem.org/mediawiki/2017/2/2c/Protein_Overexpression_.pdf and https://static.igem.org/mediawiki/2017/0/0d/October_.pdf)

"BBa_K2443001-Oct_17_gel1.png"


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1805
    Illegal SapI.rc site found at 1523