Difference between revisions of "Part:BBa K2286005"

 
 
(10 intermediate revisions by 2 users not shown)
Line 3: Line 3:
 
<partinfo>BBa_K2286005 short</partinfo>
 
<partinfo>BBa_K2286005 short</partinfo>
  
−
This part contains a root-specific promoter from Arabidopsis thaliana root and Hypocotyls. Compared to the full-length promoter, PYK10-1457 lacks the 785 bp base at the 5 'end , but its strength is increased by several times
+
This part contains a root-specific promoter from <em>Arabidopsis thaliana</em> root and Hypocotyls. Compared to the full-length promoter,  
 +
PYK10-1457 lacks the 785 bp base at the 5 'end , but its strength is increased by several times.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
Line 12: Line 13:
 
<partinfo>BBa_K2286005 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2286005 SequenceAndFeatures</partinfo>
  
 +
 +
==Characterization==
 +
 +
 +
To confirm whether the PPYK10-1457 promoter was strictly root-specific, we tested the roots and leaves of positive plants with RT-PCR.
 +
The results showed that the target gene CKX3 mainly express in the roots, only small amount of expression in leaves, indicating that
 +
PYK10-1457 is an excellent root-specific promoter.
 +
 +
[[File:T--UESTC-China--PYK10-1457-RT-PCR-2.png|800px|thumb|center|'''Fig.1''' RT-PCR of CKX3 activated by PYK10-1457.]]
 +
 +
 +
 +
'''References'''
 +
 +
1.Nitz, I., et al., Pyk10, a seedling and root specific gene and promoter from <em>Arabidopsis thaliana</em>. Plant Sci, 2001. 161(2): p. 337-346.
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Latest revision as of 17:43, 1 November 2017


PYK10-1457：a seedling and root specific promoter

This part contains a root-specific promoter from Arabidopsis thaliana root and Hypocotyls. Compared to the full-length promoter, PYK10-1457 lacks the 785 bp base at the 5 'end , but its strength is increased by several times.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

To confirm whether the PPYK10-1457 promoter was strictly root-specific, we tested the roots and leaves of positive plants with RT-PCR. The results showed that the target gene CKX3 mainly express in the roots, only small amount of expression in leaves, indicating that PYK10-1457 is an excellent root-specific promoter.

Fig.1 RT-PCR of CKX3 activated by PYK10-1457.


References

1.Nitz, I., et al., Pyk10, a seedling and root specific gene and promoter from Arabidopsis thaliana. Plant Sci, 2001. 161(2): p. 337-346.