Difference between revisions of "Part:BBa K1231000:Experience"
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== Application NAWI_Graz 2017 == | == Application NAWI_Graz 2017 == | ||
Twin part [https://parts.igem.org/Part:BBa_K2348001 BBa_K2348001] was created because information in this part was missing. Part itself works and asr promoter is activated at pH 5.0 and 4.5. | Twin part [https://parts.igem.org/Part:BBa_K2348001 BBa_K2348001] was created because information in this part was missing. Part itself works and asr promoter is activated at pH 5.0 and 4.5. | ||
Revision as of 17:39, 1 November 2017
Application NAWI_Graz 2017
Twin part BBa_K2348001 was created because information in this part was missing. Part itself works and asr promoter is activated at pH 5.0 and 4.5.
User Reviews
UNIQ1bd6101e0b764442-partinfo-00000000-QINU
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iGEM Dundee 2016 |
The function of this part was characterised in the hope of designing a pH-responsive system. We hooked up this asr promomoter to an excellent reporter system (BBa_E0840) and characterised its activity under this new composite biobrick (BBa_K1962014). The system showed some pH-dependent activity. We also hooked up the asr promoter to biobricks encoding immunity proteins towards colicins. This should give some degree of regulation of transcription of the Im-Ia immunity protein (standard biobrick BBa_K1962001 and composite part BBa_K1962015) and the Im-E3 immunity protein (standard biobrick BBa_K1962004 and composite part BBa_K1962016). |
UNIQ1bd6101e0b764442-partinfo-00000008-QINU