Difference between revisions of "Part:BBa K2381009"

 
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<partinfo>BBa_K2381009 short</partinfo>
 
<partinfo>BBa_K2381009 short</partinfo>
  
the split dCas9 is expressed along.
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It is the first part of split dCas9 which is cut at the amino acid site 713 and 714.
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<h3>References</h3>
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Nihongaki, Yuta, et al. "Photoactivatable CRISPR-Cas9 for optogenetic genome editing.." Nature Biotechnology 33.7 (2015).
  
 
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Revision as of 17:38, 1 November 2017


Conventional split dCas9-2

It is the first part of split dCas9 which is cut at the amino acid site 713 and 714.

References

Nihongaki, Yuta, et al. "Photoactivatable CRISPR-Cas9 for optogenetic genome editing.." Nature Biotechnology 33.7 (2015).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1239
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]