Difference between revisions of "Part:BBa K2225002"

 
 
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<partinfo>BBa_K2225002 short</partinfo>
 
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AtSultr1;2 for S.cerevisiae (transporter for sulfate)
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AtSultr1;2 is a high affinity sulfate transporter that naturally occurs in Arabidopsis thaliana, but is codon optimized for Saccharomyces cerevisiae.
  
 
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<partinfo>BBa_K2225002 parameters</partinfo>
 
<partinfo>BBa_K2225002 parameters</partinfo>
 
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===Design Notes===
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We optimized the codon-usage for Saccharomyces cerevisiae.
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=== Description & Source ===
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The gene encodes for a high-affinity sulfate transporter that naturally occurs in Arabidopsis thaliana and is expressed at epidermis and cortex of its roots [1].
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AtSultr1;2 belongs to a family of sulfate transporters and due to studies with mutants the AtSultr1;2 seems to be the most important one. The mutant having a AtSultr1;1-knockout showed up a phenotype similar to the wildtype while the Sultr1;2-mutant showed differences in the phenotype compared to the wildtype relating to root length, growth and sulphate uptake. This confirms the assumption that AtSultr1;2 plays a major role in the sulfate uptake system [2,3]. 
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===References===
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[1] N. Yoshimoto, E. Inoue, A. Watanabe-Takahashi et al., “Posttranscriptional regulation of high-affinity sulfate transporters in Arabidopsis by sulfur nutrition,” Plant physiology, vol. 145, no. 2, pp. 378–388, 2007.
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[2] M. Barberon, P. Berthomieu, M. Clairotte et al., “Unequal functional redundancy between the two Arabidopsis thaliana high-affinity sulphate transporters SULTR1;1 and SULTR1;2,” The New phytologist, vol. 180, no. 3, pp. 608–619, 2008.
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[3] T. Kataoka, A. Watanabe-Takahashi, N. Hayashi et al., “Vacuolar Sulfate Transporters Are Essential Determinants Controlling Internal Distribution of Sulfate in Arabidopsis,” The Plant Cell, vol. 16, no. 10, pp. 2693–2704, 2004.

Latest revision as of 17:35, 1 November 2017


AtSultr1;2 for S.cerevisiae (high-affinity sulfate transporter )

AtSultr1;2 is a high affinity sulfate transporter that naturally occurs in Arabidopsis thaliana, but is codon optimized for Saccharomyces cerevisiae.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 781
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 849
    Illegal NgoMIV site found at 1030
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1227
    Illegal BsaI.rc site found at 1503


Design Notes

We optimized the codon-usage for Saccharomyces cerevisiae.

Description & Source

The gene encodes for a high-affinity sulfate transporter that naturally occurs in Arabidopsis thaliana and is expressed at epidermis and cortex of its roots [1]. AtSultr1;2 belongs to a family of sulfate transporters and due to studies with mutants the AtSultr1;2 seems to be the most important one. The mutant having a AtSultr1;1-knockout showed up a phenotype similar to the wildtype while the Sultr1;2-mutant showed differences in the phenotype compared to the wildtype relating to root length, growth and sulphate uptake. This confirms the assumption that AtSultr1;2 plays a major role in the sulfate uptake system [2,3].

References

[1] N. Yoshimoto, E. Inoue, A. Watanabe-Takahashi et al., “Posttranscriptional regulation of high-affinity sulfate transporters in Arabidopsis by sulfur nutrition,” Plant physiology, vol. 145, no. 2, pp. 378–388, 2007.

[2] M. Barberon, P. Berthomieu, M. Clairotte et al., “Unequal functional redundancy between the two Arabidopsis thaliana high-affinity sulphate transporters SULTR1;1 and SULTR1;2,” The New phytologist, vol. 180, no. 3, pp. 608–619, 2008.

[3] T. Kataoka, A. Watanabe-Takahashi, N. Hayashi et al., “Vacuolar Sulfate Transporters Are Essential Determinants Controlling Internal Distribution of Sulfate in Arabidopsis,” The Plant Cell, vol. 16, no. 10, pp. 2693–2704, 2004.