Difference between revisions of "Part:BBa K2448006"

Latest revision as of 17:32, 1 November 2017

PsiR from Agrobacterium tumefaciens

This part is an E. coli codon optimized version of the PsiR found in Agrobacterium tumefaciens str. C58 (Atu4743).

Usage and Biology

PsiR is a predicted LacI family transcription factor with high affinity for D-Psicose [1]. This implies that PsiR is potentially capable of binding a consensus sequence in the promoter region and preventing transcription of the regulated promoters in the absence of D-Psicose, in a similar manner to the way LacI does in the absence of allolactose (or the synthetic IPTG).

Our team used this transcription factor together with the Agrobacterium tumefaciens pPsiA or pPsiR promoters (BBa_K2448010 and BBa_K2448011) as well as with the pPsiTac1 synthetic promoter (BBa_K2448016) to build three Psicose biosensors (BBa_K2448025, BBa_K2448026 and BBa_K2448027). The results show that PsiR behaved as predicted under and without induction.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 4
Illegal AgeI site found at 691
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2448006 short</partinfo>
-This part is an E. coli codon optimized version of the PsiR found in Agrobacterium tumefaciens str. C58 (Atu4743).
+This part is an ''E. coli'' codon optimized version of the PsiR found in ''Agrobacterium tumefaciens'' str. C58 (Atu4743).
 ===Usage and Biology===
-PsiR is a predicted LacI family transcription factor with high affinity for D-Psicose. This means that PsiR will prevent transcription of the regulated promoters in the absence of D-Psicose by binding the consensus sequences before the Transcription Start Site.
+PsiR is a predicted LacI family transcription factor with high affinity for D-Psicose [1]. This implies that PsiR is potentially capable of binding a consensus sequence in the promoter region and preventing transcription of the regulated promoters in the absence of D-Psicose, in a similar manner to the way LacI does in the absence of allolactose (or the synthetic IPTG).
-Our team used this transcription factor in some of our Psicose biosensors ([[Part:BBa_K2448025|BBa_K2448025]], [[Part:BBa_K2448026|BBa_K2448026]] and [[Part:BBa_K2448027|BBa_K2448027]]) and the results shows that PsiR behaved as predicted under and without induction.
+Our team used this transcription factor together with the ''Agrobacterium tumefaciens'' pPsiA or pPsiR promoters ([[Part:BBa_K2448010|BBa_K2448010]] and [[Part:BBa_K2448011|BBa_K2448011]]) as well as with the pPsiTac1 synthetic promoter ([[Part:BBa_K2448016|BBa_K2448016]]) to build three Psicose biosensors ([[Part:BBa_K2448025|BBa_K2448025]], [[Part:BBa_K2448026|BBa_K2448026]] and [[Part:BBa_K2448027|BBa_K2448027]]). The results show that PsiR behaved as predicted under and without induction.
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