Difference between revisions of "Part:BBa K1465205"
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<partinfo>BBa_K1465205 parameters</partinfo>
 
<partinfo>BBa_K1465205 parameters</partinfo>
 
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===User Reviews===
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<I>james_zhang</I>
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In 2017 SZU-China iGEM team changed the sequence of original part(<partinfo>BBa_K1465205</partinfo>) and the new part <partinfo>BBa_K2232022</partinfo> was submited.
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===iGEM2017 SZU-China===
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We add the RBS of Bacillus subtilis and a segment of signal peptide at the upstream of cds of Carboxysomal Carbonic Anhydrase(csoS3) from Halothiobacillus neapolitanus, in order to achieve the expression and secretion of this part in the B.subtilis WB800. The construction of the shuttle expression-secretion vector_CsoS3 is shown as follow in figure 1.
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<div>
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<center><html><img src='https://parts.igem.org/File:CsoS3_1.png' ></html></center>
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</div>

Revision as of 17:18, 1 November 2017

Carbonic anhydrase (csoS3) of the carboxysome of Halothiobacillus neapolitanus


The carbonic anhydrase from Halothiobacillus neapolitanus converts incoming hydrogen carbonate into carbon dioxide inside the carboxysome. This step is essential for the CO2 fixation.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 166
  • 1000
    COMPATIBLE WITH RFC[1000]


User Reviews

james_zhang

In 2017 SZU-China iGEM team changed the sequence of original part(BBa_K1465205) and the new part BBa_K2232022 was submited.

iGEM2017 SZU-China

We add the RBS of Bacillus subtilis and a segment of signal peptide at the upstream of cds of Carboxysomal Carbonic Anhydrase(csoS3) from Halothiobacillus neapolitanus, in order to achieve the expression and secretion of this part in the B.subtilis WB800. The construction of the shuttle expression-secretion vector_CsoS3 is shown as follow in figure 1.