Difference between revisions of "Part:BBa I763019:Experience"
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We cloned the part inside a high copy number plasmid (pSB1AK3. When we checked part lenght with a gel electrophoresis that was ok. Anyway, when we checked for fluorescence we observed that just some bacteria were fluorescent. We grew bacteria in M9 + lactose + IPTG (2 mM),but always we saw that not all of them were fluorescent. | We cloned the part inside a high copy number plasmid (pSB1AK3. When we checked part lenght with a gel electrophoresis that was ok. Anyway, when we checked for fluorescence we observed that just some bacteria were fluorescent. We grew bacteria in M9 + lactose + IPTG (2 mM),but always we saw that not all of them were fluorescent. | ||
| − | So, we tried tried the same cloning in a low copy number plasmid (pSB4A3), thinking that | + | So, we tried tried the same cloning in a low copy number plasmid (pSB4A3), thinking that it could better with a lower amount of cI repressor, but we obtained the same result. |
===Applications of BBa_I763019=== | ===Applications of BBa_I763019=== | ||
Revision as of 13:01, 26 October 2007
We cloned the part inside a high copy number plasmid (pSB1AK3. When we checked part lenght with a gel electrophoresis that was ok. Anyway, when we checked for fluorescence we observed that just some bacteria were fluorescent. We grew bacteria in M9 + lactose + IPTG (2 mM),but always we saw that not all of them were fluorescent.
So, we tried tried the same cloning in a low copy number plasmid (pSB4A3), thinking that it could better with a lower amount of cI repressor, but we obtained the same result.
Applications of BBa_I763019
User Reviews
UNIQ6ba29ad6fbb97047-partinfo-00000000-QINU UNIQ6ba29ad6fbb97047-partinfo-00000001-QINU