Difference between revisions of "Part:BBa K2398000"

Revision as of 17:15, 1 November 2017

Cytochrome c for the synthesis of organosilicons

We present a codon-optimized version of the cytochrome c protein derived from Rhodotermus marinus. This protein is able to perform the conversion of organosilicons. It is a novel catalytic unit that allows utilization of silicon compounds [Arnold et al., 2016]. When in its mature form, it catalyzes a carbene insertion into silicon-hydrogen bonds.

Usage and Biology

The cytochrome c is used for the catalysis of silicon compounds

Characterisation

File:Https://static.igem.org/mediawiki/parts/9/92/T--Heidelberg--GCAnilineCompound.png

Figure 1: Determination of the MIC of beta-lactamases with varying carbenicillin concentration in liquid culture. The OD600 was determined with a sample of 100 µl in 96 well plates

File:Https://static.igem.org/mediawiki/parts/1/16/T--Heidelberg--MSAnilineCompound.png

Figure 1: Determination of the MIC of beta-lactamases with varying carbenicillin concentration in liquid culture. The OD600 was determined with a sample of 100 µl in 96 well plates

File:Https://static.igem.org/mediawiki/parts/e/ef/T--Heidelberg--GCNonAninlineCompound.png

Figure 1: Determination of the MIC of beta-lactamases with varying carbenicillin concentration in liquid culture. The OD600 was determined with a sample of 100 µl in 96 well plates

File:Https://static.igem.org/mediawiki/parts/2/2b/T--Heidelberg--MSNonAnilineCompound.png

Figure 1: Determination of the MIC of beta-lactamases with varying carbenicillin concentration in liquid culture. The OD600 was determined with a sample of 100 µl in 96 well plates

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 93
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 10: / Line 10: @@
 ===Characterisation===
-[[File:https://static.igem.org/mediawiki/2017/9/92/T--Heidelberg--GCAnilineCompound.png|frame|center|Figure 1: Determination of the MIC of beta-lactamases with varying carbenicillin concentration in liquid culture. The OD600 was determined with a sample of 100 µl in 96 well plates]]
+[[File:https://static.igem.org/mediawiki/parts/9/92/T--Heidelberg--GCAnilineCompound.png|frame|center|Figure 1: Determination of the MIC of beta-lactamases with varying carbenicillin concentration in liquid culture. The OD600 was determined with a sample of 100 µl in 96 well plates]]
-[[File:https://static.igem.org/mediawiki/2017/1/16/T--Heidelberg--MSAnilineCompound.png|frame|center|Figure 1: Determination of the MIC of beta-lactamases with varying carbenicillin concentration in liquid culture. The OD600 was determined with a sample of 100 µl in 96 well plates]]
+[[File:https://static.igem.org/mediawiki/parts/1/16/T--Heidelberg--MSAnilineCompound.png|frame|center|Figure 1: Determination of the MIC of beta-lactamases with varying carbenicillin concentration in liquid culture. The OD600 was determined with a sample of 100 µl in 96 well plates]]
-[[File:https://static.igem.org/mediawiki/2017/e/ef/T--Heidelberg--GCNonAninlineCompound.png|frame|center|Figure 1: Determination of the MIC of beta-lactamases with varying carbenicillin concentration in liquid culture. The OD600 was determined with a sample of 100 µl in 96 well plates]]
+[[File:https://static.igem.org/mediawiki/parts/e/ef/T--Heidelberg--GCNonAninlineCompound.png|frame|center|Figure 1: Determination of the MIC of beta-lactamases with varying carbenicillin concentration in liquid culture. The OD600 was determined with a sample of 100 µl in 96 well plates]]
-[[File:https://static.igem.org/mediawiki/2017/2/2b/T--Heidelberg--MSNonAnilineCompound.png|frame|center|Figure 1: Determination of the MIC of beta-lactamases with varying carbenicillin concentration in liquid culture. The OD600 was determined with a sample of 100 µl in 96 well plates]]
+[[File:https://static.igem.org/mediawiki/parts/2/2b/T--Heidelberg--MSNonAnilineCompound.png|frame|center|Figure 1: Determination of the MIC of beta-lactamases with varying carbenicillin concentration in liquid culture. The OD600 was determined with a sample of 100 µl in 96 well plates]]