Difference between revisions of "Part:BBa K2348012"

Latest revision as of 16:54, 1 November 2017

alx promoter and riboswitch + mNeonGreen
This part actually is a composite part containing the alx promoter and riboswitch BBa_K2348000 and mNeonGreen BBa_K1761003. But in addition, between riboswitch and mNeonGreen a TEV-site and a f-degron are inserted. Also, mNeonGreen ins 6xHis-taged and the parts ends with a T7-terminator. Because the part does not work when build as Biobrick due to the Biobrick scar this part is listed as basic part.
TEV-site and F-degron allow a fast and controllable degradation of mNeonGreen wich, in combination with the fast maturation time of mNeonGreen, lead to a fast colour change when used together with our other pH-sensitive part BBa_K2348011

Usage and Biology

The part is used as pH regulated mNeonGreen expression device. Data of expression is shown in the alx Promoter and riboswitch part BBa_K2348000.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 312

@@ Line 5: / Line 5: @@
 TEV-site and F-degron allow a fast and controllable degradation of mNeonGreen wich, in combination with the fast maturation time of mNeonGreen, lead to a fast colour change when used together with our other pH-sensitive part [https://parts.igem.org/Part:BBa_K2348011 BBa_K2348011]
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+The part is used as pH regulated mNeonGreen expression device. Data of expression is shown in the alx Promoter and riboswitch part [https://parts.igem.org/Part:BBa_K2348000 BBa_K2348000].
 <!-- -->
 <span class='h3bb'><b>Sequence and Features</b></span><br>
 https://static.igem.org/mediawiki/2017/thumb/2/27/Alx_part_Graz.jpeg/799px-Alx_part_Graz.jpeg
 <partinfo>BBa_K2348012 SequenceAndFeatures</partinfo>