Difference between revisions of "Part:BBa K2384003"

Revision as of 16:41, 1 November 2017

DsbA+MBP(Pb)

the periplasm display protein Dsba-MBP, which binding the lead of the enviroment. Disulfide bond formation protein A (DsbA) is a powerful oxidative protein of the thioredoxin family. DsbA can help protein to fold correctly both in vivo and in vitro 。The C-terminal fusion protein of the maltose-binding protein can be efficiently and rapidly output into the periplasm as a key component of the expression of the metal-binding peptide of the cyclin.

Our sequence optimization is based on the sequence provided by Peking University's IGEM VIKI in 2010.It is more suitable for expression in Bacillus

Usage and Biology

Optimization Report

1.Codon Used Adjustment The best value is 1 for sequence optimization.

Before Codon Adjustment

After Codon Adjustment

2.Codon Used Distribution Show the relative codon used distribution

Before Optimization

After Optimization

3.GC Content: The comparison of GC content between original sequence and optimized sequence

Before Optimization

After Optimization

The expression of DsbA+MBP(Pb) detected using SDS-PAGE

Plasmid digested by EcoRI-PstI

Sequence and Features