Difference between revisions of "Part:BBa K2384004"
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<th>[[Image:T--FAFU-CHINA--DsbA-MBP123.jpg|thumb|400px|'''The expression of DsbA-MBP(Hg) detected using SDS-PAGE ''']]</th>
 
<th>[[Image:T--FAFU-CHINA--DsbA-MBP123.jpg|thumb|400px|'''The expression of DsbA-MBP(Hg) detected using SDS-PAGE ''']]</th>
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<th>[[Image:T--FAFU-CHINA--M-DSBA-MBP(Hg).png|thumb|400px|'''Plasmid digested by PvuII ''']]</th>
 
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Revision as of 16:38, 1 November 2017


DsbA+MBP(Hg)

DsbA, a protein that can export its C-terminal fusion protein into periplasm more efficiently and faster than maltose binding protein, was used as the key component to express our Metal Binding Peptide periplasmically .

Our sequence optimization is based on the sequence provided by Peking University's IGEM VIKI in 2010.It is more suitable for expression in Bacillus



Usage and Biology

Optimization Report

1.Codon Used Adjustment The best value is 1 for sequence optimization.


Before Codon Adjustment
After Codon Adjustment


2.Codon Used Distribution Show the relative codon used distribution

Before Optimization
After Optimization

3.GC Content: The comparison of GC content between original sequence and optimized sequence

Before Optimization
After Optimization
The expression of DsbA-MBP(Hg) detected using SDS-PAGE
Plasmid digested by PvuII

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 255
    Illegal BglII site found at 267
    Illegal BglII site found at 711
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]