Difference between revisions of "Part:BBa K2314106"
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This part is a gene which encoding a cellobiose transporter(CDT-1). Assimilate cellobiose into intracellular.                                                                                                         
 
This part is a gene which encoding a cellobiose transporter(CDT-1). Assimilate cellobiose into intracellular.                                                                                                         
 
In our project, we contract an engineered strain to use cellobiose. We all know that <i>S. cerevisiae</i> cannot ferment the cellodextrins naturally released by cellulases and require cellulase cocktails supplemented by β-glucosidase to quantitatively produce fermentable glucose.
 
In our project, we contract an engineered strain to use cellobiose. We all know that <i>S. cerevisiae</i> cannot ferment the cellodextrins naturally released by cellulases and require cellulase cocktails supplemented by β-glucosidase to quantitatively produce fermentable glucose.
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Cellobiose is not catabolized by <i>S. cerevisiae</i> and is not accumulated in the cytoplasm.So we express a functional cellodextrin transport system(CDT-1) from <i>N. crassa</i> allow S. cerevisiae to grow with cellobiose as the sole carbon source.                   CDT-1 is high-affinity cellobiose transporters, with Michaelis constant (Km) values of 4.0T0.3mM, respectively. Cellobiose transport by CDT-1 is inhibited by excess cello-triose, and CDT-1 activity is also inhibited by cellotetraose.                                      What's more, the combinations of CDT-1 with GH1-1 constitute fully functional cellodextrin transport systems. We have constructed an engineered bacterium that can be used as a carbon source, which can be used to produce ethanol by fermentation of fiber sugars, and the following are our experimental results.
+
Cellobiose is not catabolized by <i>S. cerevisiae</i> and is not accumulated in the cytoplasm.So we express a functional cellodextrin transport system(CDT-1) from <i>N. crassa</i> allow <i>S. cerevisiae</i> to grow with cellobiose as the sole carbon source. CDT-1 is high-affinity cellobiose transporters, with Michaelis constant (Km) values of 4.0T0.3mM, respectively. Cellobiose transport by CDT-1 is inhibited by excess cello-triose, and CDT-1 activity is also inhibited by cellotetraose.                                      What's more, the combinations of CDT-1 with GH1-1 constitute fully functional cellodextrin transport systems. We have constructed an engineered bacterium that can be used as a carbon source, which can be used to produce ethanol by fermentation of fiber sugars, and the following are our experimental results.
  
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2314515 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K2314106 SequenceAndFeatures</partinfo>
  
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K2314515 parameters</partinfo>
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<partinfo>BBa_K2314103 parameters</partinfo>
 
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Revision as of 16:29, 1 November 2017


This part is a gene which encoding a cellobiose transporter(CDT-1).

This part is a gene which encoding a cellobiose transporter(CDT-1). Assimilate cellobiose into intracellular. In our project, we contract an engineered strain to use cellobiose. We all know that S. cerevisiae cannot ferment the cellodextrins naturally released by cellulases and require cellulase cocktails supplemented by β-glucosidase to quantitatively produce fermentable glucose. Cellobiose is not catabolized by S. cerevisiae and is not accumulated in the cytoplasm.So we express a functional cellodextrin transport system(CDT-1) from N. crassa allow S. cerevisiae to grow with cellobiose as the sole carbon source. CDT-1 is high-affinity cellobiose transporters, with Michaelis constant (Km) values of 4.0T0.3mM, respectively. Cellobiose transport by CDT-1 is inhibited by excess cello-triose, and CDT-1 activity is also inhibited by cellotetraose. What's more, the combinations of CDT-1 with GH1-1 constitute fully functional cellodextrin transport systems. We have constructed an engineered bacterium that can be used as a carbon source, which can be used to produce ethanol by fermentation of fiber sugars, and the following are our experimental results.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 484
    Illegal NotI site found at 602
    Illegal NotI site found at 962
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 874
    Illegal XhoI site found at 1432
    Illegal XhoI site found at 1627
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1024
    Illegal BsaI.rc site found at 1435
    Illegal BsaI.rc site found at 1579
    Illegal SapI site found at 945