Difference between revisions of "Part:BBa K309016:Experience"

(→‎User Reviews)
 
(2 intermediate revisions by the same user not shown)
Line 15: Line 15:
 
<I>Username</I>
 
<I>Username</I>
 
|width='60%' valign='top'|
 
|width='60%' valign='top'|
−
The design of our project is based on optogenetics, trough the usage of channelrhodopsin and the calcium indicator. In order to visualize the location of injected plasmid directly before formal experiments, we require a reporter protein for location visualization.
+
Enter the review inofrmation here.
−
Nowadays, there are various fusion proteins, but we expect to gain an existent part. After searching in the iGEM database, eGFP is found out submitted by other teams which provides the basic parts for later characterization.
+
−
We have great expectation of testing and characterizing the function of eGFP due to the statement called "sample It's complicated" in the IGEM database, which means no one has characterising it. We have GFP in our lab in SUSTech-SHENZHEN University, we add introns so as to ensure the expression in C.elegans and add pre”ATG” for improvement.
+
−
The results is displayed as follows.  
+
 
|};
 
|};
 
<!-- End of the user review template -->
 
<!-- End of the user review template -->
 
<!-- DON'T DELETE --><partinfo>BBa_K309016 EndReviews</partinfo>
 
<!-- DON'T DELETE --><partinfo>BBa_K309016 EndReviews</partinfo>
 +
 +
 +
Group: iGEM17_SUSTech_Shenzhen
 +
 +
Author: iGEM17_SUSTech_Shenzhen
 +
 +
Test for GFP: localize the neuron-AWB
 +
We successfully ligated the str-1::Chrimson::GEM-GECO::GFP and injected the plasmids into C.elegans through microinjection. This picture was one of our stable inheritance strains. We used 488nm to excite AWB and observe at 500-540nm wavelength through confocal. We successfully observed a single green slender neuron on the anterior region of C.elegans. Comparing to the normal localisation of AWB, we indicated that the fluoresecence of GFP appeared in AWB. Thus we successfully used GFP to localise the position of target neuron.
 +
 +
[[File: T--SUSTech_Shenzhen--C.elegans_AWB_GFP.png|800px|thumb|left|GFP expressed in AWB|GFP expressed in AWB]]

Latest revision as of 16:24, 1 November 2017


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K309016

User Reviews

UNIQe9ea1435de36fda8-partinfo-00000000-QINU UNIQe9ea1435de36fda8-partinfo-00000001-QINU


Group: iGEM17_SUSTech_Shenzhen

Author: iGEM17_SUSTech_Shenzhen

Test for GFP: localize the neuron-AWB We successfully ligated the str-1::Chrimson::GEM-GECO::GFP and injected the plasmids into C.elegans through microinjection. This picture was one of our stable inheritance strains. We used 488nm to excite AWB and observe at 500-540nm wavelength through confocal. We successfully observed a single green slender neuron on the anterior region of C.elegans. Comparing to the normal localisation of AWB, we indicated that the fluoresecence of GFP appeared in AWB. Thus we successfully used GFP to localise the position of target neuron.

GFP expressed in AWB