Difference between revisions of "Part:BBa K302035:Experience"

Revision as of 16:21, 1 November 2017

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Please enter how you used this part and how it worked out.

Applications of BBa_K302035

User Review

iGEM2017 SZU-China confirmed the function of BBa_K302035.

We made characterization of this part in our chassis B.subtilis WB800.The sucrose was added in the culture medium when the value of OD₆₀₀ reach stationary phase initially, and the final concentration of sucrose was 20mmol/L. Then we recorded the variation of OD₆₀₀ by interval of 4 hours and the result is shown as follow.

Fig. The OD₆₀₀ of original strain WB800 and recombinant B.subtilis WB800_MazEF recorded by interval of 4 hours.The red arrow represents the adjunction of Sucrose (20mmol/L). For detail, see http://http://2017.igem.org/Team:SZU-China/Results

@@ Line 6: / Line 6: @@
 ===Applications of BBa_K302035===
-===User Reviews===
+===User Review===
+==iGEM2017 SZU-China confirmed the function of BBa_K302035.==
+We made characterization of this part in our chassis B.subtilis WB800.The sucrose was added in the culture medium when the value of OD<sub>600</sub> reach stationary phase initially, and the final concentration of sucrose was 20mmol/L. Then we recorded the variation of OD<sub>600</sub> by interval of 4 hours and the result is shown as follow.
+<div>
+<center><html><img src='https://static.igem.org/mediawiki/parts/thumb/8/81/Characterization_of_MazEF-suicide_switch_in_Bacillus_subtilis_strain_WB800.png/800px-Characterization_of_MazEF-suicide_switch_in_Bacillus_subtilis_strain_WB800.png' ></html></center>
+</div>
-https://parts.igem.org/Part:BBa_K2232009
+Fig. The OD<sub>600</sub> of original strain WB800 and recombinant B.subtilis WB800_MazEF recorded by interval of 4 hours.The red arrow represents the adjunction of Sucrose (20mmol/L).
-SZU-CHINA
+For detail, see http://http://2017.igem.org/Team:SZU-China/Results