Difference between revisions of "Part:BBa K2492000"

 
 
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<partinfo>BBa_K2492000 short</partinfo>
 
<partinfo>BBa_K2492000 short</partinfo>
  
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Modified GFP with three introns which can help well expression of GFP in C.elegans. In our group we use it to locate the neuron AWB of C.elegans. If we get successfully microinjection C.elegans, GFP will specifically high expressed in AWB with the help of special promoter str1(no biobrick part).
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This part arises from BBa_K309016[https://parts.igem.org/wiki/index.php?title=Part:BBa_K309016], which is improved and characterized by our group.
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Modified GFP with three introns which can help well expression of GFP in C.elegans. In our group, we use it to indicate the location of neuron AWB in C.elegans. We integrated str1::CoCHR::GEM-GECO::GFP transgene in C.elegans by microinjection. Modified GFP will be highly expressed in AWB driven by promoter str1(no biobrick part).
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2492000 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2492000 SequenceAndFeatures</partinfo>
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Test for GFP: localize the neuron-AWB
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We successfully ligated the str-1::Chrimson::GEM-GECO::GFP and injected the plasmids into C.elegans through microinjection. This picture was one of our stable inheritance strains. We used 488nm to excite AWB and observe at 500-540nm wavelength through confocal. We successfully observed a single green slender neuron on the anterior region of C.elegans. Comparing to the normal localisation of AWB, we indicated that the fluoresecence of GFP appeared in AWB. Thus we successfully used GFP to localise the position of target neuron.
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[[File: T--SUSTech_Shenzhen--C.elegans_AWB_GFP.png|800px|thumb|left|GFP expressed in AWB|GFP expressed in AWB]]
  
  

Latest revision as of 16:21, 1 November 2017


GFP (optimised, for use in the C. elegans chassis)

This part arises from BBa_K309016[1], which is improved and characterized by our group. Modified GFP with three introns which can help well expression of GFP in C.elegans. In our group, we use it to indicate the location of neuron AWB in C.elegans. We integrated str1::CoCHR::GEM-GECO::GFP transgene in C.elegans by microinjection. Modified GFP will be highly expressed in AWB driven by promoter str1(no biobrick part).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 797


Test for GFP: localize the neuron-AWB We successfully ligated the str-1::Chrimson::GEM-GECO::GFP and injected the plasmids into C.elegans through microinjection. This picture was one of our stable inheritance strains. We used 488nm to excite AWB and observe at 500-540nm wavelength through confocal. We successfully observed a single green slender neuron on the anterior region of C.elegans. Comparing to the normal localisation of AWB, we indicated that the fluoresecence of GFP appeared in AWB. Thus we successfully used GFP to localise the position of target neuron.


GFP expressed in AWB