Difference between revisions of "Part:BBa K2302011"

 
 
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<partinfo>BBa_K2302011 short</partinfo>
 
<partinfo>BBa_K2302011 short</partinfo>
  
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it contains three enzymes of the serine synthesis pathway. Bacteria harboring this plasmidcan be induced by arabinose to synthesize and secrete serine. A SD sequence (RBS) were added to the front of each coding sequence of serA, serB and serC.
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This plasmid cords for fatty acid binding protein and anchor it on the outer membrane, it consists four basic parts: promoter T7, His, Lpp-OmpA, and L-FABP. Lpp-OmpA is sequence cords for a functional peptide, which give efficient surface anchoring of FABP, and FABP is a kind of protein which can bind long chain fatty acid. By transforming this plasmid, a bacteria can catch long chain fatty acid in liquid and carry it on its membrane.
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===Usage and Biology===
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Latest revision as of 16:11, 1 November 2017


T7-His-Lpp-ompA-L-FABP-T7terminater

This plasmid cords for fatty acid binding protein and anchor it on the outer membrane, it consists four basic parts: promoter T7, His, Lpp-OmpA, and L-FABP. Lpp-OmpA is sequence cords for a functional peptide, which give efficient surface anchoring of FABP, and FABP is a kind of protein which can bind long chain fatty acid. By transforming this plasmid, a bacteria can catch long chain fatty acid in liquid and carry it on its membrane.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 491
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 716