Difference between revisions of "Part:BBa K2398503:Design"
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K2398503 short</partinfo>
 
<partinfo>BBa_K2398503 short</partinfo>
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This part is the coding sequence of the E. coli beta-lactamase. <br><br>
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Design:
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This part contains a fully functional beta-lactamase expression cassette. E. coli beta-lactamase is under control of the constitutive wildtype E. coli promoter. Expression is terminated by the his-operon terminator of pSB1C3. <br><br>
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In this part and related parts, mutations are introduced into the coding sequence. These mutations modify the enzyme activity (see results).
 
In this part and related parts, mutations are introduced into the coding sequence. These mutations modify the enzyme activity (see results).
  

Latest revision as of 15:47, 1 November 2017


beta-lactamase_ Y103D_M67G_F70G This part is the coding sequence of the E. coli beta-lactamase.

In this part and related parts, mutations are introduced into the coding sequence. These mutations modify the enzyme activity (see results).



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

compatible with RFC10 and Goldengate


Source

genomic, with modifications

References