Difference between revisions of "Part:BBa K2348012"

Revision as of 15:42, 1 November 2017

alx promoter and riboswitch + mNeonGreen
This part actually is a composite part containing the alx promoter and riboswitch BBa_K2348000 and mNeonGreen BBa_K1761003. But in addition, between riboswitch and mNeonGreen a TEV-site and a f-degron are inserted. Also, mNeonGreen ins 6xHis-taged and the parts ends with a T7-terminator. Because the part does not work when build as Biobrick due to the Biobrick scar this part is listed as basic part. TEV-site and f-degron allow a fast and controllable degradation of mNeonGreen and in combination with the fast maturation time of mNeonGreen allow a fast cooler change when used together with our other pH-sensitive part BBa_K2348011

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 312

@@ Line 1: / Line 1: @@
 __NOTOC__
-<partinfo>BBa_K2348012 short</partinfo>
+<partinfo>BBa_K2348012 short</partinfo><br>
+This part actually is a composite part containing the alx promoter and riboswitch [https://parts.igem.org/Part:BBa_K2348000 BBa_K2348000] and mNeonGreen [https://parts.igem.org/Part:BBa_K1761003 BBa_K1761003]. But in addition, between riboswitch and mNeonGreen a TEV-site and a f-degron are inserted. Also, mNeonGreen ins 6xHis-taged and the parts ends with a T7-terminator. Because the part does not work when build as Biobrick due to the Biobrick scar this part is listed as basic part.
+TEV-site and f-degron allow a fast and controllable degradation of mNeonGreen and in combination with the fast maturation time of mNeonGreen allow a fast cooler change when used together with our other pH-sensitive part [https://parts.igem.org/Part:BBa_K2348011 BBa_K2348011]
-?
 <!-- Add more about the biology of this part here