Difference between revisions of "Part:BBa K2522001"
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| − | + | The plasmid is engineered to detect communication signals between the pathogenic E. coli strains. The first plasmid created with PLas_sfGFP promoter is activated by 3OC12 in order to produce Green Fluorescent Protein (GFP) PLas promoter detects 3OC12, a known quorum sensing molecule. The team used this plasmid as an initial experimentation using promoter sites and the observations of positive responses to quorum sensing molecules. | |
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Latest revision as of 15:24, 1 November 2017
sfGFP produced in presence of 3OC12
The plasmid is engineered to detect communication signals between the pathogenic E. coli strains. The first plasmid created with PLas_sfGFP promoter is activated by 3OC12 in order to produce Green Fluorescent Protein (GFP) PLas promoter detects 3OC12, a known quorum sensing molecule. The team used this plasmid as an initial experimentation using promoter sites and the observations of positive responses to quorum sensing molecules.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1006
Illegal NheI site found at 1029 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1382
Illegal AgeI site found at 1579 - 1000COMPATIBLE WITH RFC[1000]