Difference between revisions of "Part:BBa K2353001:Experience"
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tsPurpleDAS was ordered and hydrated from IDT and then digested and ligated into 1C3 so that it could be sent for sequencing and then submitted. Running the digest on a gel showed tsPurpleDAS to be the correct length, however when sequenced, we discovered that the DAS degradation tag was not present. It is hypothesized that at some point during the cloning workflow, tsPurple was confused with tsPurpleDAS. | tsPurpleDAS was ordered and hydrated from IDT and then digested and ligated into 1C3 so that it could be sent for sequencing and then submitted. Running the digest on a gel showed tsPurpleDAS to be the correct length, however when sequenced, we discovered that the DAS degradation tag was not present. It is hypothesized that at some point during the cloning workflow, tsPurple was confused with tsPurpleDAS. | ||
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<img src="https://static.igem.org/mediawiki/2017/5/58/Tspurples_gel.jpeg" width="400px"> | <img src="https://static.igem.org/mediawiki/2017/5/58/Tspurples_gel.jpeg" width="400px"> | ||
Revision as of 15:23, 1 November 2017
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UNIQ7ba0612dc29d6443-partinfo-00000000-QINU
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tsPurpleDAS was ordered and hydrated from IDT and then digested and ligated into 1C3 so that it could be sent for sequencing and then submitted. Running the digest on a gel showed tsPurpleDAS to be the correct length, however when sequenced, we discovered that the DAS degradation tag was not present. It is hypothesized that at some point during the cloning workflow, tsPurple was confused with tsPurpleDAS.
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UNIQ7ba0612dc29d6443-partinfo-00000003-QINU