Difference between revisions of "Part:BBa K2353001:Experience"

 
(→‎User Reviews)
Line 8: Line 8:
 
===User Reviews===
 
===User Reviews===
 
<!-- DON'T DELETE --><partinfo>BBa_K2353001 StartReviews</partinfo>
 
<!-- DON'T DELETE --><partinfo>BBa_K2353001 StartReviews</partinfo>
−
<!-- Template for a user review
 
 
{|width='80%' style='border:1px solid gray'
 
{|width='80%' style='border:1px solid gray'
 
|-
 
|-
 
|width='10%'|
 
|width='10%'|
−
<partinfo>BBa_K2353001 AddReview number</partinfo>
+
<partinfo>BBa_K2353001 Review 1</partinfo>
−
<I>Username</I>
+
<I>jleveille</I>
 
|width='60%' valign='top'|
 
|width='60%' valign='top'|
−
Enter the review inofrmation here.
+
tsPurpleDAS was ordered and hydrated from IDT and then digested and ligated into 1C3 so that it could be sent for sequencing and then submitted. Running the digest on a gel showed tsPurpleDAS to be the correct length, however when sequenced, we discovered that the DAS degradation tag was not present. It is hypothesized that at some point during the cloning workflow, tsPurple was confused with tsPurpleDAS.
 +
<html>
 +
<img src="https://static.igem.org/mediawiki/2017/5/58/Tspurples_gel.jpeg" width="400px">
 +
</html>
 +
<br>
 +
<i style="font-size:12px;"> Gel results from a digest of all three tsPurple parts. From top to bottom: BBa_K2353000, BBa_K2353002, BBa_K2353001 </i>
 
|};
 
|};
−
<!-- End of the user review template -->
 
 
<!-- DON'T DELETE --><partinfo>BBa_K2353001 EndReviews</partinfo>
 
<!-- DON'T DELETE --><partinfo>BBa_K2353001 EndReviews</partinfo>

Revision as of 15:21, 1 November 2017


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K2353001

User Reviews

UNIQa83f37badddb3470-partinfo-00000000-QINU

BBa_K2353001 Review 1 Not understood jleveille

tsPurpleDAS was ordered and hydrated from IDT and then digested and ligated into 1C3 so that it could be sent for sequencing and then submitted. Running the digest on a gel showed tsPurpleDAS to be the correct length, however when sequenced, we discovered that the DAS degradation tag was not present. It is hypothesized that at some point during the cloning workflow, tsPurple was confused with tsPurpleDAS.
Gel results from a digest of all three tsPurple parts. From top to bottom: BBa_K2353000, BBa_K2353002, BBa_K2353001

;

UNIQa83f37badddb3470-partinfo-00000003-QINU