Difference between revisions of "Part:BBa K2448001"
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mEmerald is a derivative of the Green Fluorescent Protein from ''Aequorea victoria'' with improved features in terms of intrinsic brightness, photostability, resistance to bleaching, maturation and folding at 37°C [1]. The protein contains six mutations compared to wild-type: F64L, S65T, S72A, N149K, M153T, I167T & A206K [2]. | mEmerald is a derivative of the Green Fluorescent Protein from ''Aequorea victoria'' with improved features in terms of intrinsic brightness, photostability, resistance to bleaching, maturation and folding at 37°C [1]. The protein contains six mutations compared to wild-type: F64L, S65T, S72A, N149K, M153T, I167T & A206K [2]. | ||
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| + | Figure 1 illustrates the intense colour of mEmerald expressing ''E. coli'' cells. | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
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Our team used this codon optimized mCherry to build the Universal Biosensing Chassis ([[Part:BBa_K2448023|BBa_K2448023]]). | Our team used this codon optimized mCherry to build the Universal Biosensing Chassis ([[Part:BBa_K2448023|BBa_K2448023]]). | ||
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| + | [[File:T--Evry_Paris-Saclay--UBC_LBplate_noIPTG_noXgal.jpg|300px]] | ||
| + | Figure 1. Picture of an LB-chloramphenicol plate containing ''E. coli '' colonies transformed with [[Part:BBa_K2448023|BBa_K2448023]]. | ||
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Revision as of 14:18, 1 November 2017
mEmerald
mEmerald is a derivative of the Green Fluorescent Protein from Aequorea victoria with improved features in terms of intrinsic brightness, photostability, resistance to bleaching, maturation and folding at 37°C [1]. The protein contains six mutations compared to wild-type: F64L, S65T, S72A, N149K, M153T, I167T & A206K [2].
Figure 1 illustrates the intense colour of mEmerald expressing E. coli cells.
Usage and Biology
Applications are various: Fusions, promoter & secretion studies, FRET and other quantitative imaging techniques.
Our team used this codon optimized mCherry to build the Universal Biosensing Chassis (BBa_K2448023).
Figure 1. Picture of an LB-chloramphenicol plate containing E. coli colonies transformed with BBa_K2448023.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 28
Illegal AgeI site found at 151 - 1000COMPATIBLE WITH RFC[1000]