Difference between revisions of "Part:BBa K2448004:Experience"
 
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===Applications of BBa_K2448004===
 
===Applications of BBa_K2448004===
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mCherry is a fluorescent monomeric protein widely used in biotechnology. Like the majority of red fluorescent proteins, it is derived from the Red Fluorescent protein from Discosoma sp. mCherry is sometimes preferred over other fluorophores due to its color, its rapid maturation time (t0.5=15 min), its lower brightness (around 50% of eGFP) as well as its improved photostability and resistance to bleaching. Applications are various: Fusions, promoter & secretion studies, FRET and other quantitative imaging techniques.
 
  
 
Our team used this codon optimized mCherry to build the Universal Biosensing Chassis ([[Part:BBa_K2448023|BBa_K2448023]] and [[Part:BBa_K2448024|BBa_K2448024]]) and test our Psicose Biosensors ([[Part:BBa_K2448025|BBa_K2448025]], [[Part:BBa_K2448026|BBa_K2448026]], [[Part:BBa_K2448027|BBa_K2448027]], [[Part:BBa_K2448028|BBa_K2448028]], [[Part:BBa_K2448029|BBa_K2448029]], [[Part:BBa_K2448030|BBa_K2448030]] and [[Part:BBa_K2448031|BBa_K2448031]]) as well as our Fructose Biosensor ([[Part:BBa_K2448032|BBa_K2448032]]).
 
Our team used this codon optimized mCherry to build the Universal Biosensing Chassis ([[Part:BBa_K2448023|BBa_K2448023]] and [[Part:BBa_K2448024|BBa_K2448024]]) and test our Psicose Biosensors ([[Part:BBa_K2448025|BBa_K2448025]], [[Part:BBa_K2448026|BBa_K2448026]], [[Part:BBa_K2448027|BBa_K2448027]], [[Part:BBa_K2448028|BBa_K2448028]], [[Part:BBa_K2448029|BBa_K2448029]], [[Part:BBa_K2448030|BBa_K2448030]] and [[Part:BBa_K2448031|BBa_K2448031]]) as well as our Fructose Biosensor ([[Part:BBa_K2448032|BBa_K2448032]]).
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[[File:T--Evry_Paris-Saclay--BBa_K2448032_with_Fru_liquid.jpg|250px]] [[File:T--Evry_Paris-Saclay--BBa_K2448032_with_Fru_pellet.jpg|207px]]
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Figure 1.  mCherry expression in ''E. coli'' cells harbouring [[Part:BBa_K2448032|BBa_K2448032]] in the presence of Fructose.
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 14:07, 1 November 2017


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K2448004

Our team used this codon optimized mCherry to build the Universal Biosensing Chassis (BBa_K2448023 and BBa_K2448024) and test our Psicose Biosensors (BBa_K2448025, BBa_K2448026, BBa_K2448027, BBa_K2448028, BBa_K2448029, BBa_K2448030 and BBa_K2448031) as well as our Fructose Biosensor (BBa_K2448032).

T--Evry Paris-Saclay--BBa K2448032 with Fru liquid.jpg T--Evry Paris-Saclay--BBa K2448032 with Fru pellet.jpg

Figure 1. mCherry expression in E. coli cells harbouring BBa_K2448032 in the presence of Fructose.

User Reviews

UNIQ852d1003d929cb42-partinfo-00000000-QINU UNIQ852d1003d929cb42-partinfo-00000001-QINU