Difference between revisions of "Part:BBa K1979001"
 
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The sequence codes for cellulose binding domain cbh2 in prokaryotic cells.
 
The sequence codes for cellulose binding domain cbh2 in prokaryotic cells.
 
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<h3>Contribution by iGEM 2017 SDSZ-China</h3>
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<h3>Contribution - iGEM 2017 SDSZ-China</h3>
 
<h4>Proof of the CBD function</h4>
 
<h4>Proof of the CBD function</h4>
  

Latest revision as of 13:55, 1 November 2017


CBD (cbh2)

The sequence codes for cellulose binding domain cbh2 in prokaryotic cells.

Contribution - iGEM 2017 SDSZ-China

Proof of the CBD function

The resulted solution after expression is mixed with a gauze piece, and the green color on gauze is recorded. Then the gauze is washed three times using ddH2O, but the green fluorescent on it is not significantly reduced, proving that the CDB is well functioned. In comparison, no green fluorescent is left after washing the gauze mixed with GFP-ChBD (Chintin binding domain).

Cbd-before.jpeg

[Fig. 1. GFP-CBD on gauze before washing]

Cbd-after.jpeg

[Fig. 2. GFP-CBD on gauze after washing]

Chbd-before.jpeg

[Fig. 3. GFP-ChBD on gauze before washing]

Chbd-after.jpeg

[Fig. 4. GFP-ChBD on gauze after washing]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 11