Difference between revisions of "Part:BBa K2205025"
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| − | <p>This part was made to | + | <p>This part was made to test the modularity of the system by inserting the PSP3 Phage Sensitivity Tuner (BBa_K274381) construct between the two parts of the empty processing module of the Sensynova platform. </p> |
<p>The part BBa_ K274381 is under the control of our connector 1, part BBa_K2205010, while controlling our connector 2, part BBa_K2205011. </p> | <p>The part BBa_ K274381 is under the control of our connector 1, part BBa_K2205010, while controlling our connector 2, part BBa_K2205011. </p> | ||
Revision as of 13:52, 1 November 2017
Sensynova Framework (PSP3 pag signal tuner Processing Module - pCell)
Description
The part BBa_K2205025 was designed as Processor unit of the Sensynova multicellular framework for biosensors development (Figure 1).
This part was made to test the modularity of the system by inserting the PSP3 Phage Sensitivity Tuner (BBa_K274381) construct between the two parts of the empty processing module of the Sensynova platform.
The part BBa_ K274381 is under the control of our connector 1, part BBa_K2205010, while controlling our connector 2, part BBa_K2205011.
This system is normally repressed expect in the presence of connector 1 subsequently activating the transcription of connector 2 (C12 – RHL) triggering the reporter modules of the platform.
References
iGEM Community. (2009). Team Cambridge 2009. [online] Available at: http://2009.igem.org/Team:Cambridge [Accessed 30 Oct. 2017].
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 383
Illegal AgeI site found at 580 - 1000COMPATIBLE WITH RFC[1000]