Difference between revisions of "Part:BBa K2507002"
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TtrS/R are two basic parts which belong to the two-component systems. This system is from marine Shewanella species. After validate this system in laboratory Escherichia coli, this system can function as a tetrathionate sensor and reporter. Because of tetrathionate is an indicator of gut inflammation, this system can be used as a sensor of gut inflammation. Connect TtrR with sfGFP or chromoprotein， this system can response to tetrathionate by produce protein.
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<i>E. coli</i>-codon-optimized TtrS(BBa_K2507002) and TtrR (BBa_K2507003) are two basic parts which are derived from the two-component system of the marine bacterium <i>Shewanella baltica.</i> TtrS is the membrane-bound sensor kinase (SK) which can sense tetrathionate outside the cell, and TtrR is the DNA-binding response regulator (RR).  
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Revision as of 13:41, 1 November 2017

TtrS

Background

E. coli-codon-optimized TtrS(BBa_K2507002) and TtrR (BBa_K2507003) are two basic parts which are derived from the two-component system of the marine bacterium Shewanella baltica. TtrS is the membrane-bound sensor kinase (SK) which can sense tetrathionate outside the cell, and TtrR is the DNA-binding response regulator (RR).



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1309
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 783
  • 1000
    COMPATIBLE WITH RFC[1000]



Results