Difference between revisions of "Part:BBa K2398508:Design"

 
Line 4: Line 4:
  
 
<partinfo>BBa_K2398508 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2398508 SequenceAndFeatures</partinfo>
 +
 +
 +
This part contains a fully functional beta-lactamase expression cassette. E. coli beta-lactamase is under control of the constitutive wildtype E. coli promoter. Expression is terminated by the his-operon terminator of pSB1C3. <br><br>
 +
In this part and related parts, mutations are introduced into the coding sequence. These mutations modify the enzyme activity (see results).
  
  

Revision as of 13:34, 1 November 2017


beta-lactamase_P164G


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


This part contains a fully functional beta-lactamase expression cassette. E. coli beta-lactamase is under control of the constitutive wildtype E. coli promoter. Expression is terminated by the his-operon terminator of pSB1C3.

In this part and related parts, mutations are introduced into the coding sequence. These mutations modify the enzyme activity (see results).


Design Notes

compatible with RFC10


Source

genomic with modifications

References