Difference between revisions of "Part:BBa K2387010"
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More information can be found <a href="http://2017.igem.org/Team:Wageningen_UR/Results/SpecificVisualization#optimalactivation">here</a>
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More information can be found <a href="http://2017.igem.org/Team:Wageningen_UR/Results/SpecificVisualization#results">here</a>
  
 
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Latest revision as of 13:07, 1 November 2017


CpxA-eYFPn[1-154] fusion + araC/pBAD promoter

The [http://parts.igem.com/Part:BBa_K2387005 N-terminus of eYFP] is fused to transmembrane signal transducer [http://parts.igem.com/Part:BBa_K2387002 CpxA] in order to visualize the activation of the Cpx pathway. The fusion is put under the control of the L-arabinose inducible araC/pBAD promoter. Strong RBS BBa_B0034 is used to regulate transcription.

CpxA and eYFPn were linked together using a [http://parts.igem.com/Part:BBa_K1486004 Flexible Linker] consisting of two times the amino acids GGGGS.

It can be used in combination with the fusion [http://parts.igem.com/Part:BBa_K2387011 CpxR-eYFPc[155-238]] to visualize the activation of the Cpx pathway via Bimolecular Fluorescence Complementation


Usage and Biology

This BioBrick (in combination with BBa_K2387011) was used following this protocol.

Figure 1: CpxA-CpxR protein interaction visualized with different L-arabinose concentrations over time.

More information can be found here



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961