Difference between revisions of "Part:BBa K2387010"

 
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<partinfo>BBa_K2387010 short</partinfo>
 
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The [http://parts.igem.com/Part:BBa_K2387007 N-terminus of eYFP] is fused to transmembrane signal transducer [http://parts.igem.com/Part:BBa_K2387002 CpxA] in order to visualize the activation of the Cpx pathway. The fusion is put under the control of the L-arabinose inducible [https://parts.igem.org/Part:BBa_I0050 araC/pBAD promoter]. Strong RBS [https://parts.igem.org/Part:BBa_B0034 BBa_B0034] is used to regulate transcription.
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The [http://parts.igem.com/Part:BBa_K2387005 N-terminus of eYFP] is fused to transmembrane signal transducer [http://parts.igem.com/Part:BBa_K2387002 CpxA] in order to visualize the activation of the Cpx pathway. The fusion is put under the control of the L-arabinose inducible [https://parts.igem.org/Part:BBa_I0050 araC/pBAD promoter]. Strong RBS [https://parts.igem.org/Part:BBa_B0034 BBa_B0034] is used to regulate transcription.
  
 
CpxA and eYFPn were linked together using a [http://parts.igem.com/Part:BBa_K1486004 Flexible Linker] consisting of two times the amino acids GGGGS.
 
CpxA and eYFPn were linked together using a [http://parts.igem.com/Part:BBa_K1486004 Flexible Linker] consisting of two times the amino acids GGGGS.
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===Usage and Biology===
 
===Usage and Biology===
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This BioBrick (in combination with BBa_K2387011) was used following this <a href="https://static.igem.org/mediawiki/2017/d/d4/Cpx_Dimerization.pdf"> protocol</a>.
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<div class="figure-center">
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                                                <img class="figure-center" src="https://static.igem.org/mediawiki/parts/6/60/T--Wageningen_UR--CpxAR_interaction_araCupdated.png" width="500">
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                                                <div class="figure-center-caption">
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                                                    <b>Figure 1:</b> CpxA-CpxR protein interaction visualized with different L-arabinose concentrations over time.
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                                            </div>
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More information can be found <a href="http://2017.igem.org/Team:Wageningen_UR/Results/SpecificVisualization#results">here</a>
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Latest revision as of 13:07, 1 November 2017


CpxA-eYFPn[1-154] fusion + araC/pBAD promoter

The [http://parts.igem.com/Part:BBa_K2387005 N-terminus of eYFP] is fused to transmembrane signal transducer [http://parts.igem.com/Part:BBa_K2387002 CpxA] in order to visualize the activation of the Cpx pathway. The fusion is put under the control of the L-arabinose inducible araC/pBAD promoter. Strong RBS BBa_B0034 is used to regulate transcription.

CpxA and eYFPn were linked together using a [http://parts.igem.com/Part:BBa_K1486004 Flexible Linker] consisting of two times the amino acids GGGGS.

It can be used in combination with the fusion [http://parts.igem.com/Part:BBa_K2387011 CpxR-eYFPc[155-238]] to visualize the activation of the Cpx pathway via Bimolecular Fluorescence Complementation


Usage and Biology

This BioBrick (in combination with BBa_K2387011) was used following this protocol.

Figure 1: CpxA-CpxR protein interaction visualized with different L-arabinose concentrations over time.

More information can be found here



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961