Difference between revisions of "Part:BBa K2353000:Design"

 
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===Design Notes===
 
===Design Notes===
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The promoter and terminator are both part of different parts, which means tspurple is not expressed unless it is assembled with pLambdaR-LacI and r0011ClpXPCI.
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tsPurple is intended to be assembled with pLambdaR-LacI (which is the promoter and regulatory system) and pLac-ClpXP-CI (which is the non-lysosomal protease). The terminator is located at the beginning of pLac-ClpXP-CI. When fully assembled, p-lambda-r LacI transcribes tsPurple and LacI represses pLac, preventing transcription of ClpXP-CI. Upon induction with IPTG, LacI binds to IPTG which prevents repression of pLac. Therefore, ClpXP-CI is transcribed; theoretically this should not change the levels of expression of tsPurple since there is no degradation tag attached. The levels of expression of tsPurple with degradation tags can then be compared to the level of tsPurple expression (as a base-line comparison).
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IMPORTANT NOTE: The promoter and terminator are both part of different parts, which means tspurple is not expressed unless it is assembled with pLambdaR-LacI (BBa_K1911000).
  
  

Revision as of 12:42, 1 November 2017


tsPurple


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

tsPurple is intended to be assembled with pLambdaR-LacI (which is the promoter and regulatory system) and pLac-ClpXP-CI (which is the non-lysosomal protease). The terminator is located at the beginning of pLac-ClpXP-CI. When fully assembled, p-lambda-r LacI transcribes tsPurple and LacI represses pLac, preventing transcription of ClpXP-CI. Upon induction with IPTG, LacI binds to IPTG which prevents repression of pLac. Therefore, ClpXP-CI is transcribed; theoretically this should not change the levels of expression of tsPurple since there is no degradation tag attached. The levels of expression of tsPurple with degradation tags can then be compared to the level of tsPurple expression (as a base-line comparison).
IMPORTANT NOTE: The promoter and terminator are both part of different parts, which means tspurple is not expressed unless it is assembled with pLambdaR-LacI (BBa_K1911000).


Source

This part is composed from sequences found in the parts registry.

References