Difference between revisions of "Part:BBa K2447013"

Latest revision as of 11:36, 1 November 2017

Temperature-sensitive repressible system

In this construct, the temperature sensitive TlpA36 protein will be produced with the addition of a upstream promoter (as specified by the user), and depending on the temperatures, it will either form Tlpa36 dimers that are capable of repressing the pTlpA promoter (in temperatures below 36 degrees) or stay in monomeric form (above 36 degrees) which makes the protein unable to repress the promoter.

BBA_K2447013 has been characterised using BBa_K2447014(which is the characterisation construct). Experimental results of this IPTG-inducible temperature system with GFP reporter (BBa_K2447014) that was transformed into 10β cells had demonstrated that our temperature system was fully functional. From Figure 1, when the temperature was 36 °C and higher (‘ON’ switch condition), GFP production was not repressed. However, when temperature was below 36 °C (‘OFF’ switch condition), GFP production was significantly repressed.

For more information on our experimental result and characterization protocol, do click [http://2017.igem.org/Team:NUS_Singapore/Experiments here].

For more information on how do we improve the overall characterization process by automation, do click [http://2017.igem.org/Team:NUS_Singapore/Measurement here].

Figure 1: Bba_K2447014 is the characterisation construct for our temperature-sensitive repressive system (Bba_K2447013). GFP/OD_600nm is shown here for transformed 10β cells after 24 hours incubation at various temperatures.

Sequence and Features