Difference between revisions of "Part:BBa K2447013"

Revision as of 11:26, 1 November 2017

Temperature-sensitive repressible system

In this construct, the temperature sensitive TlpA36 protein will be produced with the addition of a upstream promoter (as specified by the user), and depending on the temperatures, it will either form Tlpa36 dimers that are capable of repressing the pTlpA promoter (in temperatures below 36 degrees) or stay in monomeric form (above 36 degrees) which makes the protein unable to repress the promoter.

BBA_K2447013 has been characterised using BBa_K2447014(which is the characterisation construct). Experimental results of this IPTG-inducible temperature system with GFP reporter (BBa_K2447014) had demonstrated that our temperature system is fully functional.

Figure 1: Bba_K2447014 is the characterisation construct for our temperature-sensitive repressive system. GFP/OD600nm is shown here after 24 hours incubation at various temperatures.

For more information on our experimental result and characterization protocol, do click [http://2017.igem.org/Team:NUS_Singapore/Experiments here].

For more information on how do we improve the overall characterization process by automation, do click [http://2017.igem.org/Team:NUS_Singapore/Measurement here].

Sequence and Features