Difference between revisions of "Part:BBa K2448033"
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This part is an ''E. coli'' codon optimized version of the D-Psicose 3-epimerase (Dpe) from ''Clostridium cellulolyticum'' str. ATCC 35319
 
This part is an ''E. coli'' codon optimized version of the D-Psicose 3-epimerase (Dpe) from ''Clostridium cellulolyticum'' str. ATCC 35319
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(Uniprot B8I944, [[Part:BBa_K2448021|BBa_K2448021]]).equipped with a strong RBS ([[Part:BBa_K2448019|BBa_K2448019]]) and placed under the control of the pTacI promoter ([[Part:BBa_K864400|BBa_K864400]]).
+
(Uniprot B8I944, [[Part:BBa_K2448021|BBa_K2448021]]) equipped with a strong RBS ([[Part:BBa_K2448019|BBa_K2448019]]) and placed under the control of the pTacI promoter ([[Part:BBa_K864400|BBa_K864400]]).
  
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 11:09, 1 November 2017


D-Psicose 3-epimerase (Dpe) from Clostridium cellulolyticum under the control of pTacI promoter

D-Psicose 3-epimerase (Dpe) from Clostridium cellulolyticum

This part is an E. coli codon optimized version of the D-Psicose 3-epimerase (Dpe) from Clostridium cellulolyticum str. ATCC 35319 (Uniprot B8I944, BBa_K2448021) equipped with a strong RBS (BBa_K2448019) and placed under the control of the pTacI promoter (BBa_K864400).

Usage and Biology

D-Psicose 3-epimerase (EC: 5.1.3.30) catalyses the reversible epimerization of D-fructose into D-psicose, D-psicose being the carbon-3 (C3) epimer of D-fructose.

T--Evry Paris-Saclay--EC 5.1.3.30.png

The C. cellulolyticum Dpe is a 33.034 kDa protein of 293 amino acids.

This enzyme is highly specific for D-psicose, but it is also able to catalyse with very low activity the epimerization of D-tagatose [1].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 714
  • 1000
    COMPATIBLE WITH RFC[1000]