Difference between revisions of "Part:BBa K2398500"
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This part consists of a beta-lactamase expression cassette. The coding sequence was modified with point mutations, which modulate enzyme activity.<br><br>
 
This part consists of a beta-lactamase expression cassette. The coding sequence was modified with point mutations, which modulate enzyme activity.<br><br>
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===Usage and Biology===
  
 
<b>Determination of the Minimal Inhibitory Concentration:</b>
 
<b>Determination of the Minimal Inhibitory Concentration:</b>
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Figure 2: Determination of the MIC for the eleven best beta-lactamase candidates with higher carbenicillin. The OD600 was determined with a sample of 100 µl in 96 well plates|
 
Figure 2: Determination of the MIC for the eleven best beta-lactamase candidates with higher carbenicillin. The OD600 was determined with a sample of 100 µl in 96 well plates|
  
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===Usage and Biology===
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Revision as of 11:01, 1 November 2017


Beta-lactamase promoter from E. coli

This part consists of a beta-lactamase expression cassette. The coding sequence was modified with point mutations, which modulate enzyme activity.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    INCOMPATIBLE WITH RFC[23]
    Unknown
  • 25
    INCOMPATIBLE WITH RFC[25]
    Unknown
  • 1000
    COMPATIBLE WITH RFC[1000]