Difference between revisions of "Part:BBa K2448050"

Revision as of 10:37, 1 November 2017

pPsiTac2

This part is a hybrid synthetic promoter composed of the -35 and the Pribnow box sequences of pTacI promoter (BBa_K864400) [1] and two consensus binding site of PsiR regulator of Rhizobiale [2].

Usage and Biology

D-Psicose inducible promoters naturally occur in nature, but they are quite long and not well characterized. In order to assess the efficiency of our psicose biosensor(s), we engineered a strong promoter, pTacI (BBa_K864400), regulated by LacI to make it psicose inducible.

pPsiTac2 is a synthetic promoter similar to pPsiTac1 (BBa_K2448016). It is derived from pTacI (BBa_K864400) from which we removed the LacO sequence recognized by LacI and replaced it by the consensus binding sequence of a transcription factor, PsiR interacting with psicose (BBa_K2448006). In pPsiTac2 compared to pPsiTac1 (BBa_K2448016) there is a second binding site for PsiR upstream the -35 box.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 D-Psicose inducible promoters naturally occur in nature, but they are quite long and not well characterized. In order to assess the efficiency of our psicose biosensor(s), we engineered a strong promoter, pTacI ([[Part:BBa_K864400|BBa_K864400]]), regulated by LacI to make it psicose inducible.
-pPsiTac2 is a synthetic promoter similar to [[Part:BBa_K2448016|BBa_K2448016]]. It is derived from pTacI ([[Part:BBa_K864400|BBa_K864400]]) from which we removed the LacO sequence recognized by LacI and replaced it by the consensus binding sequence of a transcription factor, PsiR interacting with psicose ([[Part:BBa_K2448006|BBa_K2448006]]). In pPsiTac2 (compared to [[Part:BBa_K2448016|BBa_K2448016]]) there is a second binding site for PsiR upstream the -35 box.
+pPsiTac2 is a synthetic promoter similar to pPsiTac1 ([[Part:BBa_K2448016|BBa_K2448016]]). It is derived from pTacI ([[Part:BBa_K864400|BBa_K864400]]) from which we removed the LacO sequence recognized by LacI and replaced it by the consensus binding sequence of a transcription factor, PsiR interacting with psicose ([[Part:BBa_K2448006|BBa_K2448006]]). In pPsiTac2 compared to pPsiTac1 ([[Part:BBa_K2448016|BBa_K2448016]]) there is a second binding site for PsiR upstream the -35 box.
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