Difference between revisions of "Part:BBa K2205018"
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<partinfo>BBa_K2205018 short</partinfo>
 
<partinfo>BBa_K2205018 short</partinfo>
  
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Description here
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===Description===
 
<p>This part was made to testing the modularity of the system by replacing the sfGFP reporter module of the Sensynova platform design with the chromoprotein variants spisPink (BBa_K1033925). </p>
 
<p>This part was made to testing the modularity of the system by replacing the sfGFP reporter module of the Sensynova platform design with the chromoprotein variants spisPink (BBa_K1033925). </p>
  
 
<p>The spisPink protein is a pink chromoprotein extracted from the coral Stylophora pistillata. It was first extracted and characterized by Alieva <i>et al</i>. under the name spisCP (GenBank: ABB17971.1) and codon optimized for <i>E. coli</i> by Genscript. The protein has an absorption maximum at 560 nm giving it a pink colour visible to the naked eye. The strong colour is readily observed in both LB or on agar plates after less than 24 hours of incubation.</p>
 
<p>The spisPink protein is a pink chromoprotein extracted from the coral Stylophora pistillata. It was first extracted and characterized by Alieva <i>et al</i>. under the name spisCP (GenBank: ABB17971.1) and codon optimized for <i>E. coli</i> by Genscript. The protein has an absorption maximum at 560 nm giving it a pink colour visible to the naked eye. The strong colour is readily observed in both LB or on agar plates after less than 24 hours of incubation.</p>
  
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===References===
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[http://www.ncbi.nlm.nih.gov/pubmed/18648549] Alieva, Naila O., et al. "Diversity and evolution of coral fluorescent proteins." PLoS One 3.7 (2008): e2680.
  
 
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Revision as of 10:30, 1 November 2017


Sensynova Framework (Connector 2B + spisPink (Complete) - rCell)

Description

This part was made to testing the modularity of the system by replacing the sfGFP reporter module of the Sensynova platform design with the chromoprotein variants spisPink (BBa_K1033925).

The spisPink protein is a pink chromoprotein extracted from the coral Stylophora pistillata. It was first extracted and characterized by Alieva et al. under the name spisCP (GenBank: ABB17971.1) and codon optimized for E. coli by Genscript. The protein has an absorption maximum at 560 nm giving it a pink colour visible to the naked eye. The strong colour is readily observed in both LB or on agar plates after less than 24 hours of incubation.


References

[http://www.ncbi.nlm.nih.gov/pubmed/18648549] Alieva, Naila O., et al. "Diversity and evolution of coral fluorescent proteins." PLoS One 3.7 (2008): e2680.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 301
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 776