Difference between revisions of "Part:BBa K2205018"
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Description here | Description here | ||
<p>This part was made to testing the modularity of the system by replacing the sfGFP reporter module of the Sensynova platform design with the chromoprotein variants spisPink (BBa_K1033925). </p> | <p>This part was made to testing the modularity of the system by replacing the sfGFP reporter module of the Sensynova platform design with the chromoprotein variants spisPink (BBa_K1033925). </p> | ||
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<p>The spisPink protein is a pink chromoprotein extracted from the coral Stylophora pistillata. It was first extracted and characterized by Alieva <i>et al</i>. under the name spisCP (GenBank: ABB17971.1) and codon optimized for <i>E. coli</i> by Genscript. The protein has an absorption maximum at 560 nm giving it a pink colour visible to the naked eye. The strong colour is readily observed in both LB or on agar plates after less than 24 hours of incubation.</p> | <p>The spisPink protein is a pink chromoprotein extracted from the coral Stylophora pistillata. It was first extracted and characterized by Alieva <i>et al</i>. under the name spisCP (GenBank: ABB17971.1) and codon optimized for <i>E. coli</i> by Genscript. The protein has an absorption maximum at 560 nm giving it a pink colour visible to the naked eye. The strong colour is readily observed in both LB or on agar plates after less than 24 hours of incubation.</p> | ||
Revision as of 10:20, 1 November 2017
Sensynova Framework (Connector 2B + spisPink (Complete) - rCell)
Description here
This part was made to testing the modularity of the system by replacing the sfGFP reporter module of the Sensynova platform design with the chromoprotein variants spisPink (BBa_K1033925).
The spisPink protein is a pink chromoprotein extracted from the coral Stylophora pistillata. It was first extracted and characterized by Alieva et al. under the name spisCP (GenBank: ABB17971.1) and codon optimized for E. coli by Genscript. The protein has an absorption maximum at 560 nm giving it a pink colour visible to the naked eye. The strong colour is readily observed in both LB or on agar plates after less than 24 hours of incubation.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 301
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 776