Difference between revisions of "Part:BBa K2294007"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | This promoter is build up fully modular. The promoter starts with an GAL4 binding site derived from <i>GAL1</i>. After this trnaskription factor binding site a neutral spacer is placed to avoid steric hindrances between GAL4 and the TATA-box-binding- proteins of the preinitiation complex. | + | This promoter is build up fully modular. The promoter starts with an GAL4 binding site derived from <i>GAL1</i>. After this trnaskription factor binding site a neutral spacer is placed to avoid steric hindrances between GAL4 and the TATA-box-binding- proteins of the preinitiation complex. Following the TATA-box there is the core1 consiting of 30 random nucleotides. Whose porpose is as spacer between the TATA-box and the TSS sequence. In yeast the minimal gap between TATA-box and TSS should be 30 nucleotides. TSS, core1 and TATA-box are the minimal elements for an active promoter but without an additional activation element ( in this case the GAL4 binding site) the activity of the minmal promoter would be to low for efficient protein expression. <br> |
| + | All information taken from Redden 2015. | ||
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Revision as of 10:05, 1 November 2017
Synthtic minimal Galactose induced promoter + Kozak sequence
A short synthtetic promoter which can be induced by the addition of Galactose. This promoter contains already a Kozak sequence for the expression of proteins. The promoter is only 160 bp long.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
This promoter is build up fully modular. The promoter starts with an GAL4 binding site derived from GAL1. After this trnaskription factor binding site a neutral spacer is placed to avoid steric hindrances between GAL4 and the TATA-box-binding- proteins of the preinitiation complex. Following the TATA-box there is the core1 consiting of 30 random nucleotides. Whose porpose is as spacer between the TATA-box and the TSS sequence. In yeast the minimal gap between TATA-box and TSS should be 30 nucleotides. TSS, core1 and TATA-box are the minimal elements for an active promoter but without an additional activation element ( in this case the GAL4 binding site) the activity of the minmal promoter would be to low for efficient protein expression.
All information taken from Redden 2015.
Promoter characterization
The BOKU-Vienna Team 2017 submitted and characterized this part by measuring the GFP expression using flow cytometry. For detais go to the Experience page.
References
Redden, H., & Alper, H. S. (2015). The development and characterization of synthetic minimal yeast promoters. Nature Communications, 6, 7810. https://doi.org/10.1038/ncomms8810