Difference between revisions of "Part:BBa K2507016"

Revision as of 09:59, 1 November 2017

J23109-ttrR-PttrB185-BBa_K592009

Usage and Biology

E.coli codon optimized TtrS(BBa_K2507002) and TtrR(BBa_K2507003) are two basic parts which belong to the two-component system from marine Shewanella baltica. TtrS is the membrane-bound sensor kinase(SK) which can sense tetrathionate outside the cell and TtrR is the DNA-binding response regulator(RR).PttrB185-269 (BBa_K2507019) is a minimal TtrR activated promoter when TtrR is phosphorylated by TtrS after TtrS sensing tetrathionate. Winter et.al have shown that reactive oxygen species (ROS) produced by the host during inflammation convert thiosulfate to tetrathionate, which this pathogen consumes to establish a startpoint for infection (Winter et al, 2010).Thus, tetrathionate may correlate with pro-inflammation conditions and can be used as gut inflammation sensor.

Sequence and Features

Characterization

After validate this system in laboratory Escherichia coli Top10 and E.coli Nissle 1917, this system can function as a tetrathionate sensor and reporter.

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Figure 1. Schematic of ligand-induced signaling through TtrS/R and plasmid design of the sensor components. TtrS/R were tested under the situation BBa_K2507006 was in pSB4K5 backbone and BBa_K2507016 was in pSB1C3 backbone. We submitted the parts all to the iGEM registry in pSB1C3.

Reference

Daeffler, K. N., Galley, J. D., Sheth, R. U., Ortiz‐Velez, L. C., Bibb, C. O., & Shroyer, N. F., et al. (2017). Engineering bacterial thiosulfate and tetrathionate sensors for detecting gut inflammation. Molecular Systems Biology, 13(4), 923.