Difference between revisions of "Part:BBa K2194002"
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<partinfo>BBa_K2194002 short</partinfo>
 
<partinfo>BBa_K2194002 short</partinfo>
  
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The PgntK promoter is a native <i>Escherichia coli</i> negative feedback membrane stress promoter. BioBrick BBa_K2194002 contains the 200 bp upstream of the <i>gntK</i> gene in <i>Escherichia coli</i>, which has been previously experimentally shown to incorporate the full <i>Pgntk</i>promoter region. [1]
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The <i>Pgntk</i> promoter is a native <i>Escherichia coli</i> negative feedback membrane stress promoter. BioBrick BBa_K2194002 contains the 200 bp upstream of the <i>gntK</i> gene in <i>Escherichia coli</i>, which has been previously experimentally shown to incorporate the full <i>Pgntk</i> promoter region. [1]
  
  

Revision as of 09:59, 1 November 2017


PgntK Negative Feedback Membrane Stress Promoter

The Pgntk promoter is a native Escherichia coli negative feedback membrane stress promoter. BioBrick BBa_K2194002 contains the 200 bp upstream of the gntK gene in Escherichia coli, which has been previously experimentally shown to incorporate the full Pgntk promoter region. [1]


Biology

[1] Boyarskiy, Sergey et al. “Transcriptional Feedback Regulation of Efflux Protein Expression for Increased Tolerance to and Production of N-Butanol.” Metabolic Engineering 33 (2016): 130–137. Web. 28 Oct. 2017.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 14
    Illegal BsaI.rc site found at 216