Difference between revisions of "Part:BBa K2194002"

Revision as of 09:57, 1 November 2017

PgntK Negative Feedback Membrane Stress Promoter

The PgntK promoter is a native Escherichia coli negative feedback membrane stress promoter. BioBrick BBa_K2194002 contains the 200 bp upstream of the gntK gene in Escherichia coli, which has been previously experimentally shown to incorporate the full Pgntkpromoter region. [1]

Usage and Biology

[1] Boyarskiy, Sergey et al. “Transcriptional Feedback Regulation of Efflux Protein Expression for Increased Tolerance to and Production of N-Butanol.” Metabolic Engineering 33 (2016): 130–137. Web. 28 Oct. 2017.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 14
Illegal BsaI.rc site found at 216

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 The PgntK promoter is a native <i>Escherichia coli</i> negative feedback membrane stress promoter. BioBrick BBa_K2194002 contains the 200 bp upstream of the <i>gntK</i> gene in <i>Escherichia coli</i>, which has been previously experimentally shown to incorporate the full <i>Pgntk</i>promoter region. [1]
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+[1] Boyarskiy, Sergey et al. “Transcriptional Feedback Regulation of Efflux Protein Expression for Increased Tolerance to and Production of N-Butanol.” Metabolic Engineering 33 (2016): 130–137. Web. 28 Oct. 2017.
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