Difference between revisions of "Part:BBa K2309022:Experience"

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===Applications of BBa_K2309022===
 
===Applications of BBa_K2309022===
 
This year we use dot blot method to evaluate part K2309022 work.
 
This year we use dot blot method to evaluate part K2309022 work.
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The picture shows the dot blotting result of produced LL-37 which induced by IPTG in BL21 strain. The left one shows the peptide produced and right is negative contral group.
 
  
 
https://static.igem.org/mediawiki/parts/8/82/Dot_blot_for_LL-37_XJTLU-CHINA.jpeg
 
https://static.igem.org/mediawiki/parts/8/82/Dot_blot_for_LL-37_XJTLU-CHINA.jpeg
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 +
The picture shows the dot blotting result of produced LL-37 which induced by IPTG in BL21 strain. The left one shows the peptide LL-37 produced and right is non-induced BL21 by IPTG.
 +
 +
For LL-37 ability assay, we tested Minimal Inhibitory concentration(MIC) of <i>Staphylococcus aureus</i>, the result is shown as follows:
 +
 +
https://static.igem.org/mediawiki/parts/5/5c/LL-37_MIC_XJTLU-CHINA.jpeg
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 +
As picture shows that the concentration of LL-37 from 5ug/ml to 10ug/ml, the LL-37 has highly activities <i>Staphylococcus aureus</i>.
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 +
We also tested the anti-biofilm ability of LL-37 on biofilm formation of <i>Staphylococcus aureus</i>, the result as follows:
 +
 +
https://static.igem.org/mediawiki/parts/6/6c/LL-37_biofilm_XJTLU-CHINA.jpeg
 +
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The result shows that the value of Absorbance at 595nm decreases while the concentration of LL-37 increases. It seems that LL-37 peptide inhibits <i>Staphylococcus aureus</i> to form the biofilm.
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 09:48, 1 November 2017


Applications of BBa_K2309022

This year we use dot blot method to evaluate part K2309022 work.

Dot_blot_for_LL-37_XJTLU-CHINA.jpeg

The picture shows the dot blotting result of produced LL-37 which induced by IPTG in BL21 strain. The left one shows the peptide LL-37 produced and right is non-induced BL21 by IPTG.

For LL-37 ability assay, we tested Minimal Inhibitory concentration(MIC) of Staphylococcus aureus, the result is shown as follows:

LL-37_MIC_XJTLU-CHINA.jpeg

As picture shows that the concentration of LL-37 from 5ug/ml to 10ug/ml, the LL-37 has highly activities Staphylococcus aureus.

We also tested the anti-biofilm ability of LL-37 on biofilm formation of Staphylococcus aureus, the result as follows:

LL-37_biofilm_XJTLU-CHINA.jpeg

The result shows that the value of Absorbance at 595nm decreases while the concentration of LL-37 increases. It seems that LL-37 peptide inhibits Staphylococcus aureus to form the biofilm.

User Reviews

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