Difference between revisions of "Part:BBa K2294007"
Mbaumschabl (Talk | contribs) |
Mbaumschabl (Talk | contribs) (→Usage and Biology) |
||
| Line 13: | Line 13: | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | This promoter is build up fully modular. | + | This promoter is build up fully modular. The promoter starts with an GAL4 binding site derived from <i>GAL1</i>. |
<!-- --> | <!-- --> | ||
| + | |||
===Promoter characterization=== | ===Promoter characterization=== | ||
The BOKU-Vienna Team 2017 submitted and characterized this part by measuring the GFP expression using flow cytometry. For detais go to the [[Part:BBa_K2294007:Experience|Experience page]]. | The BOKU-Vienna Team 2017 submitted and characterized this part by measuring the GFP expression using flow cytometry. For detais go to the [[Part:BBa_K2294007:Experience|Experience page]]. | ||
Revision as of 08:09, 1 November 2017
Synthtic minimal Galactose induced promoter + Kozak sequence
A short synthtetic promoter which can be induced by the addition of Galactose. This promoter contains already a Kozak sequence for the expression of proteins. The promoter is only 160 bp long.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
This promoter is build up fully modular. The promoter starts with an GAL4 binding site derived from GAL1.
Promoter characterization
The BOKU-Vienna Team 2017 submitted and characterized this part by measuring the GFP expression using flow cytometry. For detais go to the Experience page.