Difference between revisions of "Part:BBa K2294007"
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A short synthtetic promoter which can be induced by the addition of Galactose. This promoter contains already a Kozak sequence for the expression of proteins. The promoter is only 160 bp long. | A short synthtetic promoter which can be induced by the addition of Galactose. This promoter contains already a Kozak sequence for the expression of proteins. The promoter is only 160 bp long. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 08:04, 1 November 2017
Synthtic minimal Galactose induced promoter + Kozak sequence
A short synthtetic promoter which can be induced by the addition of Galactose. This promoter contains already a Kozak sequence for the expression of proteins. The promoter is only 160 bp long.
Usage and Biology
This promoter is build up fully modular.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Promoter characterization
The BOKU-Vienna Team 2017 submitted and characterized this part by measuring the GFP expression using flow cytometry. For detais go to the Experience page.