Difference between revisions of "Part:BBa K2433004"

 
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<partinfo>BBa_K2433004 short</partinfo>
 
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The VirB1 promoter is an inducible promoter for virB1 that was taken from an Agrobacterium Tumefaciens plasmid. This promoter is induced by acetosyringone and VirB1 is involved in virulence in wild type A. Tumefaciens. Wounded plants release acetosyringone, causing A. Tumefaciens to upregulate the expression of VirB1 when near a wound. In the context of synthetic biology, the VirB1 promoter is a useful tool to control gene expression. For example, in A. Tumefaciens the virB1 promoter could be used to keep a potentially toxic synthetic gene like CRISPR quiescent until the bacteria neared a plant wound. Functional virG and virA are also required for virB induction. VirG and virA are both inducible by acetosyringone.
  
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The VirB1 promoter was cloned into two backbones (K135010 and J04650) containing red fluorescent protein and transformed into the Agrobacterium Tumefaciens strain GV3101 for further testing.
 
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===Usage and Biology===
 
===Usage and Biology===

Revision as of 07:55, 1 November 2017


VirB1

The VirB1 promoter is an inducible promoter for virB1 that was taken from an Agrobacterium Tumefaciens plasmid. This promoter is induced by acetosyringone and VirB1 is involved in virulence in wild type A. Tumefaciens. Wounded plants release acetosyringone, causing A. Tumefaciens to upregulate the expression of VirB1 when near a wound. In the context of synthetic biology, the VirB1 promoter is a useful tool to control gene expression. For example, in A. Tumefaciens the virB1 promoter could be used to keep a potentially toxic synthetic gene like CRISPR quiescent until the bacteria neared a plant wound. Functional virG and virA are also required for virB induction. VirG and virA are both inducible by acetosyringone.

The VirB1 promoter was cloned into two backbones (K135010 and J04650) containing red fluorescent protein and transformed into the Agrobacterium Tumefaciens strain GV3101 for further testing. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 118